Research Interests

Similar inflammatory, immunologic, and pathophysiologic events characterize disease processes at mucosal sites such as the intestine and the lung. Critical issues to this research area include understanding the role of immune cells and mediators in the production of disease. The overall aim of my research is to define microbial-epithelial and immune cell interactions in these systems, and how such interactions may affect gastrointestinal and pulmonary physiology, responsiveness, and inflammation, in an attempt to develop novel therapeutic strategies.

Courses Taught

Graduate Students

Awards

2011: Faculty of Science Award for Excellence in Research, University of Calgary
2008: Robert A. Wardle Medal, Canadian Society of Zoologists, for outstanding contributions to Parasitology research on the national and international scenes
2008: Endeavour Research Fellowship Award, Government of Australia, for research and innovation
2007: Research Excellence Award, Canadian Association of Gastroenterologists
2007: Synergy Award for collaborative research and innovation, Natural Sciences and Engineering Research Council of Canada (NSERC)
2007 - Research Excellence Award, Canadian Association of Gastroenterology
2006 - NSERC Synergy Award for collaborative research and innovation, Univ. of Calgary nominee
2005 - Students' Union Teaching Excellence Award Honorable Mention Winner
2003 - University of Calgary Graduate Students' Association Teaching Excellence

 

Selected publications

• Selected publications (from the list of the past 6 years):
  Lapointe, T.K., O’Connor, P.M., Buret, A.G. (2009) The role of epithelial malfunction in the pathogenesis of EPEC-induced diarrhea. Lab. Invest. (in press)

  kalischuk, L.D., inglis, g.d., BURET.A.G.  (2009) Plasma membrane cholesterol modulates Campylobacter jejuni induced translocation of non-invasive Escherichia coli. Gut Pathog. 1;2:1-10.

  LAMB-ROSTESKI , J.M., KALISCHUK, L.D., INGLIS, G.D., BURET, A.G. (2008) Epidermal growth factor inhibits Campylobacter jejuni-induced claudin-4 disruption, loss of epithelial barrier function, and Escherichia coli translocation. Infect. Immun. 76;8:3390-3398

  O’HARA, J., BURET, A.G. (2008) Mechanisms of intestinal tight junctional disruption during infection. Front. Biosci. (1;13):7008-7021

  FLYNN A.N., BURET, A.G. (2008) Tight junctional disruption and apoptosis in an in vitro model of Citrobacter rodentium infection. Microbial Pathogenesis. 45:98-104

  MRSNY, R.J., BROWN, G.T., GERNER-SMIDT, K., BURET, A.G., MEDDINGS, J.B., QUAN, C., KOVAL M., NUSRAT, A. (2008) A key claudin extracellular loop domain is critical for epithelial barrier integrity.  Am. J. Pathol. 172(4):905-915

  YU, L.C.H., HUANG, C-Y, KUO, W-T, SAYER, H., TURNER, J.R., BURET, A.G. (2008) SGLT-1 –mediated glucose uptake protects human intestinal epithelial cells against Giardia duodenalis –induced apoptosis. Int. J. Parasitol. 38: 923-934.

  BURET A.G. (2008) Pathophysiology of enteric infections with Giardia duodenalis. Parasite 15:261-266

  FLYNN, A.N., BURET, A,G. (2008) Caspases 3, 8, and 9 are required for induction of epithelial cell apoptosis by enteropathogenic E. coli but are dispensable for increased paracellular permeability. Microbial pathogenesis. 44(4):311-319.

  GILLRIE, M.R., KRISHNEGOWDA, G., LEE, K., BURET, A.G., ROBBINS, S.M., LOOAREESUWAN, S., GOWDA, D.C., HO, M. (2007) Src family kinase-dependent disruption of endothelial barrier function by Plasmodium falciparum merozoite proteins. Blood 110(9): 3426-3435.

  KALISCHUK, L.D., INGLIS, G.D., BURET, A.G. (2007) Strain-dependent induction of epithelial cell oncosis by Campylobacter jejuni correlates with invasion ability and is independent of cytolethal distending toxin. Microbiology 153: 2952-2963

  YU, L.C.H., TURNER, J.R., BURET, A.G. (2006). LPS/CD14 activation triggers SGLT-1 –mediated glucose uptake and cell rescue in enterocytes via early apoptotic pathways upstream of caspase-3. Exp. Cell Res. 312(17):3276-3286

  WALLACE, J.L., BURET, A.G. (2006)  GI-sparing anti-inflammatory drugs: A promising future . Gastroenterol. Hepatol. Ann. Rev. 1:123-127

  FEDWICK, J.P., LAPOINTE, T.K., MEDDINGS, J.B., SHERMAN, P.M., BURET, A.G. (2005) Helicobacter pylori activates myosin light chain kinase and disrupts claudins –4 and -5 to increase epithelial permeability in a strain-dependent fashion. Infect. Immun. 73(12);7844-7852.

  YU, L.C.H., FLYNN, A.N., TURNER, J.R., BURET, A.G. (2005) SGLT-1 mediated glucose uptake protects intestinal epithelial cells against LPS-induced apoptosis and barrier defects: A novel cellular rescue mechanism?  FASEB J. 19: 1822-1835

  SIGALET,D.L., MARTIN, G.R., BUTZNER, D., BURET, A.G., MEDDINGS, J. (2005) A pilot study of the use of epidermal growth factor in pediatric short bowel syndrome. J. Ped. Surgery 40(5):763-768

  NERLAND, E.M., LEBLANC, J.M., FEDWICK, J.P., MORCK, D.W., MERRILL, J.K., DICK, P., PARADIS, M.A., BURET, A.G. (2005) Oral Tilmicosin induces leukocyte apoptosis, reduces Leukotriene B4, and attenuates inflammation in the Actinobacillus pleuropneumoniae-Infected Porcine Lung. Am. J. Vet Res. 66(1):100-107.

  FLYNN, A.N., BURET, A.G. (2004) Proteinase-Activated Receptor 1 (PAR-1) and cell apoptosis. Apoptosis 9(6):755-763
  SCOTT, K.G.E., YU, L.C.H., BURET, A.G. (2004) The role of CD8+ and CD4+ T lymphocytes in jejunal mucosal injury during murine giardiasis. Infect. Immun. 72(6):3536-3542.

  CHIN, A.C., VERGNOLLE, N., MACNAUGHTON, W.K., WALLACE, J.L., HOLLENBERG, M.D., BURET, A.G. (2003) Proteinase-activated receptor-1 activation induces epithelial apoptosis and increases intestinal permeability. Proc. Nat. Acad. Sc. 100:11104-11109